Gene description for MFGE8
Gene name	milk fat globule EGF and factor V/VIII domain containing
Gene symbol	MFGE8
Other names/aliases	BA46 EDIL1 HMFG HsT19888 MFG-E8 MFGM OAcGD3S SED1 SPAG10 hP47
Species	Homo sapiens
Experiment description of studies that identified MFGE8 in Vascular smooth muscle cells
1 Experiment ID 2012 Identified molecule protein Extracellular vesicle type Large extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs Isolation/purification methods Centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
2 Experiment ID 2013 Identified molecule protein Extracellular vesicle type Medium extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
3 Experiment ID 2014 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
4 Experiment ID 2015 Identified molecule protein Extracellular vesicle type Large extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs Isolation/purification methods Centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
5 Experiment ID 2016 Identified molecule protein Extracellular vesicle type Medium extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
6 Experiment ID 2017 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
7 Experiment ID 2018 Identified molecule protein Extracellular vesicle type Large extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs - early passage Isolation/purification methods Centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
8 Experiment ID 2019 Identified molecule protein Extracellular vesicle type Medium extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs - early passage Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
9 Experiment ID 2020 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs - early passage Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -
10 Experiment ID 2021 Identified molecule protein Extracellular vesicle type Large extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs - late passage Isolation/purification methods Centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
11 Experiment ID 2022 Identified molecule protein Extracellular vesicle type Medium extracellular vesicles MISEV Large EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs - late passage Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting EV-TRACK -
12 Experiment ID 2023 Identified molecule protein Extracellular vesicle type Small extracellular vesicles MISEV Small EVs Identification method Western blotting PubMed ID 36433666    Organism Homo sapiens Experiment description Vascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization Authors Whitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM. Journal name Aging Cell Publication year 2023 Sample Vascular smooth muscle cells Sample name Aorta-derived VSMCs - late passage Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry EV-TRACK -